Isolation and characterization of novel EST-derived genic markers in Pisum sativum (Fabaceae)1
نویسندگان
چکیده
UNLABELLED PREMISE OF THE STUDY Novel markers were developed for pea (Pisum sativum) from pea expressed sequence tags (ESTs) having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. • METHODS AND RESULTS Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. • CONCLUSIONS The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris) demonstrates their potential for use in closely related species.
منابع مشابه
Development and characterization of 41 novel EST-SSR markers for Pisum sativum (Leguminosae).
PREMISE OF THE STUDY Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Pisum sativum for further use in genetic studies and breeding programs. METHODS AND RESULTS Forty-one novel EST-SSR primers were developed and characterized for size polymorphism in 32 Pisum sativum individuals from four populations from China. In each population, the number of all...
متن کاملDevelopment and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae)1
UNLABELLED PREMISE OF THE STUDY Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. • METHODS AND RESULTS P...
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